Orthopedic Basic Science MCQs (Part 3): Bone Physiology & Biomechanics | AAOS & ABOS Board Review 2026

Morphologic and anatomic studies confirm the pedicle is smaller on the concave side of thoracic curves. The dura is also closer to the pedicle on the concave side of the curves. Liljenqvist U, Allkemper T, Hackenberg L, et al: Analysis of vertebral morphology in idiopathic scoliosis with use of magnetic resonance imaging and multiplanar reconstruction. J Bone Joint Surg Am 2002;84:359-368.

Stress-strain plots allow easy comparison of a number of important mechanical properties, including elastic modulus (the slope of the initial straight line portion of the curve) and yield stress (the stress at the break in the curves for bone, steel, and titanium alloy). Important considerations here are much lower modulus and ultimate stress of bone and cement compared to the two metallic alloys, the fact that titanium is lower modulus but higher strength than stainless steel, and the identification of cement as the only brittle material among the four. Burstein AH, Wright TM: Fundamentals of Orthopaedic Biomechanics. Baltimore, MD, Williams and Wilkins, 1994, pp 97-129.

Bisphosphonates have been reported to reduce the incidence of new osseous lesions and prevent an increase in size of existing lesions. Zoledronic acid has been reported in clinical trials to decrease the skeletal complications of patients with multiple myeloma and with bone metastases from solid tumors. Results also have demonstrated that zoledronic acid delays the initial onset of bone complications by more than 2 months in patients with non-small-cell lung cancer and other solid tumors. In two placebo-controlled clinical studies of zoledronic acid conducted in patients with bone metastases from prostate cancer or other solid tumors, there was a decrease in the number of patients with skeletal-related events compared to placebo, and the time to the first skeletal-related event was delayed. Mundy GR, Yoneda T: Bisphosphonates as anticancer drugs. N Engl J Med 1998;339:398-400.

IGF-I, formerly known as somatomedin-C, possibly acts by both paracrine and endocrine hormone pathways. The products of the GH-IGF-I system induce proliferation without maturation of the growth plate and thus induce linear skeletal growth. The action of the thyroid hormone axis is via an active metabolite that enters target cells and signals a nuclear receptor to stimulate both proliferation and maturation of the growth plate. Increased amounts of the active steroid hormone metabolite promote proliferation and maturation of the growth plate. Calcitonin inhibits bone resorption. Binder G, Grauer ML, Wehner AV, et al: Outcome in tall stature: Final height and psychological aspects in 220 patients with and without treatment. Eur J Pediatr 1997;156:905-910. Wang J, Zhou J, Cheng CM, et al: Evidence supporting dual, IGF-I-independent and IGF-I-dependent, roles for GH in promoting longitudinal bone growth. Endocrinol 2004;180:247-255.

During osteolysis, IL-6 is secreted by fibroblasts in the membrane surrounding the prosthesis. IL-6 also can be secreted by osteoblasts in other settings, but they are not the predominant source of IL-6 in particle-induced osteolysis. The remaining cells are not major sources of IL-6.

Nonsurgical modalities remain the mainstay for treatment of herniated disks. Spontaneous resorption of herniated disks frequently is detected by MRI. Marked infiltration by macrophages and neovascularization are observed on histologic examination of herniated disks, and the resorption is believed to be related to this process. Many cytokines such as vascular endothelial growth factor, tumor necrosis factor-alpha, and metalloproteinases have been implicated in this process, but none has been found to be singularly responsible. Haro H, Kato T, Kamori H, et al: Vascular endothelial growth factor (VEGF)-induced angiogenesis in herniated disc resorption. J Orthop Res 2002;20:409-415.

Intravenous fluid administration is the best first step to treat the hypercalcemia of malignancy. Many of these patients are dehydrated, and the increased serum calcium impairs the ability of the kidney to concentrate the urine. The decreased glomerular filtration rate secondary to the hypovolemia also leads to increased tubular resorption of calcium. The establishment of normovolemia will help promote increased urinary excretion of calcium. Lasix can also be used to help promote calciuria. Mithramycin is an antibiotic derived from Streptomyces plicatus. It is part of a group of drugs referred to as chromomycin antibiotics and is the only one of this group used clinically in the United States. It is rarely used in cancer chemotherapy because of its toxicity. A number of drug-related deaths have occurred from the use of mithramycin. Its use is now limited to the treatment of hypercalcemia associated with malignancy where it is used in lower dosage than that used for the treatment of tumors. Methotrexate has no role in the treatment of hypercalcemia of malignancy. While intravenous bisphosphonates are helpful in slowing progression of metastases and may help lower cerum calcium, they are not appropriate in the emergent treatment of hypercalcemia in the metastatic cancer patient.

Lubricin and SZP share a similar primary structure but may differ in posttranslational modifications with O-linked oligosaccharides. The primary physiologic function of SZP appears to be boundary lubrication. SZP does not influence interleukin-1, collagenase, or TIMP directly because these proteins are associated with articular cartilage turnover. Hlavacek M: The influence of the acetabular labrum seal, intact articular superficial zone and synovial fluid thixotropy on squeeze-film lubrication of a spherical synovial joint. J Biomech 2002;35:1325-1335.

The stretching of the graft occurs over time as the graft is loaded. Time-dependent deformation under load is called creep and is common in viscoelastic materials such as ligament tissue. Creep can occur under both static and cyclic load conditions; time-dependent deformation will occur as long as load is applied to the tissue. Similarly, when a graft is initially tensioned to a given deformation at surgery, the load generated in the graft will decrease over time; this behavior is called stress relaxation and also is indicative of a viscoelastic material. Water content may affect the viscoelastic properties by changing the friction between collagen fibers, but studies have shown little difference in water content between grafts and normal ligaments. Fatigue failures may manifest themselves through damage to the ligament tissue, but this would require higher loads than are routinely experienced by grafts. Elastic stretch is recoverable and, therefore, does not contribute to a permanent stretch. Similarly, gross failure at the attachment would not cause a stretch, but rather a catastrophic instantaneous instability. Boorman RS, Thornton GM, Shrive NG, et al: Ligament grafts become more susceptible to creep within days after surgery. Acta Orthop Scand 2002;73:568-574. Buckwalter JA, Einhorn TA, Simon SR (eds): Orthopaedic Basic Science: Biology and Biomechanics of the Musculoskeletal System, ed 2. Rosemont, IL, American Academy of Orthopaedic Surgeons, 2000, pp 596-609.

The TcPO2 measures the O2 delivering capacity of the local vasculature. Values above 40 mm Hg have been shown to correlate with positive healing potential. The hemoglobin A1c is a good indicator of long-term glucose levels; however, it has no direct correlation with wound healing potential. Serum albumin is an indirect measure of nutritional status, and deficiencies in nutrition must be addressed before any surgery. Adequate hemoglobin levels are also necessary to promote adequate oxygenation to the amputation site. The ankle-brachial index may be falsely elevated as a result of calcified vessels in patients with diabetes mellitus. Wyss CR, Harrington RM, Burgess EM, et al: Transcutaneous oxygen tension as a predictor of success after amputation. J Bone Joint Surg Am 1988;70:203-207.

Highly cross-linked polyethylene has gained widespread acceptance for joint arthroplasty components because of reported experimental and early clinical accounts of significant reductions in wear. Cross-linking is increased by imparting additional energy into the polymer (above that conventionally used for sterilization). The thermal treatments after cross-linking stabilize the material against oxidative degradation by quenching free radicals and also reduce the elastic modulus. One disadvantage of the increased cross-linking is a reduction in toughness that makes the polyethylene more susceptible to crack initiation and propagation. The reduced toughness raises concerns for gross component fracture and fracture at stress concentrations that can arise with the locking mechanisms used to secure polyethylene inserts into metallic backings. Nonconsolidated polyethylene particles have been associated with increased subsurface density secondary to oxidative degradation in conventional polyethylene implants. The quenching of free radicals by thermal treatment in highly cross-linked polyethylene should prevent this problem. Collier JP, Currier BH, Kennedy FE, et al: Comparison of cross-linked polyethylene materials for orthopaedic applications. Clin Orthop 2003;414:289-304. Buckwalter JA, Einhorn TA, Simon SR (eds): Orthopaedic Basic Science: Biology and Biomechanics of the Musculoskeletal System, ed 2. Rosemont, IL, American Academy of Orthopaedic Surgeons, 2000, pp 203-208.

Cell surface glycoproteins present in the heterogeneous population of the cells within the graft are primarily responsible for the antigenicity. Macromolocules of the matrix have also been implicated. Cryopreserved grafts have less antigenicity than fresh. Freezing, freeze-drying, or chemical sterilization and antigen extraction of the bone allograft have all been shown to reduce the antigenicity of the graft. Freeze-drying of retroviral-infected cortical bone and tendon does not inactivate retrovirus. Immunosuppression has been shown to decrease response. Hematopoietic elements along with osteogenic, chondrogenic, fibrous, and vascular cells have been shown to be antigenic. Crawford MJ, Swenson CL, Arnoczky SP, et al: Lyophilization does not inactivate infectious retrovirus in systemically infected bone and tendon allografts. Am J Sports Med 2004;32:580-586. Stevenson S, Li XQ, Davy DT, et al: Critical biological determinants of incorporation of non-vascularized cortical bone grafts: Quantification of a complex process and structure. J Bone Joint Surg Am 1997;79:1-16.

Camptomelic dysplasia is caused by a heterozygous loss of function of the Sox9 gene. The alternatives have genetic causes, but are not linked to Sox9. Cleidocranial dysplasia is related to a defect in Cbfa-1 (Osf-2, Runx2). Schmid metaphyseal chondrodysplasia is related to Type X collagen. Fibrous dysplasia is related to a defect in the alpha subunit of stimulatory guanine-nucleotide-binding protein (Gs). Achondroplasia is related to a defect in fibroblast growth factor receptor 3. Wagner T, Wirth J, Meyer J, et al: Autosomal sex reversal and camptomelic dysplasia are caused by mutations in and around the SRY-related gene SOX9. Cell 1994;79:1111-1120. Buckwalter JA, Einhorn TA, Simon SR (eds): Orthopaedic Basic Science: Biology and Biomechanics of the Musculoskeletal System, ed 2. Rosemont, IL, American Academy of Orthopaedic Surgeons, 2000, pp 111-131.

Mineralization occurs at the site of hole zones between the collagen fibrils. Crystals begin from the necessary ions of the lattice that come together with the correct orientation to form the first stable crystal. Formation of this critical nucleus is the most energy-demanding step of crystallization. Enzymes within the extracellular matrix vesicles degrade inhibitors such as adenosine triphosphate, pyrophosphate, and proteoglycans found in the surrounding extracellular matrix. Bone mineral consists of numerous impurities (carbonate, magnesium) that are more soluble, allowing the bone to act as a reservoir for calcium, phosphate, and magnesium ions. Crystals may form by addition of ions or ion clusters to the critical nucleus in many directions, with 'kink' sites forming to branch and exponentially proliferate the crystals. Macromolecules facilitate formation of the critical nucleus and increasing local concentrations of necessary ions. Once the crystals are formed and proliferating, macromolecules bind to the surface and block the growth of the crystal, regulating size, shape, and number of crystals. Lian JB, Stein GS, Canalis E, et al: Bone formation: Osteoblast lineage cells, growth factors, matrix proteins, and the mineralization process, in Favus MJ (ed): Primer on Metabolic Bone Diseases and Disorders of Mineral Metabolism, ed 4. Philadelphia, PA, Lippincott Williams & Wilkins, 1999, pp 14-29.

Disk nutrition occurs via diffusion through pores in the end plates. The disk has no direct blood supply, and the anulus is not porous to allow diffusion. The dorsal root ganglion does not provide blood supply to the disc. Biyani A, Andersson GB: Low back pain: Pathophysiology and management. J Am Acad Orthop Surg 2004;12:106-115. Urban JG, Holm S, Maroudas A, et al: Nutrition of the intervertebral disc: Effect of fluid flow on solute transport. Clin Orthop 1982;170:296-302.

A complete answer necessarily includes the means and standard deviations of bone mineral density in both groups. Given these, which are the basic results of the study, the P-value can be calculated if desired. All of the other options preclude assessment of the actual data, that is, the information collected by the study. P-values and confidence intervals should be perceived as additional information, which help to assess the certainty of relating the study's findings to the general population, but they should not be reported instead of the results (ie, the means and standard deviations).

4b 4c The radiographs reveal small ossified masses around the femoral neck. The CT scans also show these masses and suggest that they are separate from the underlying cortex of the femoral neck, although they abut it. The MRI scan does not reveal significant marrow changes in the proximal femur apart from some mild reactive changes immediately adjacent to the nodules. These findings suggest a synovial or joint-based disorder as opposed to a primary bone tumor. The most likely diagnosis is synovial osteochondromatosis, which is consistent with the patient's mechanical symptoms. Crotty JM, Monu JU, Pope TL Jr: Synovial osteochondromatosis. Radiol Clin North Am 1996;34:327-342.

Advances in development of new tumor markers and techniques of antigen retrieval have enhanced the sensitivity and reliability of identifying the primary source of metastasis. New markers such as CK7, CK20, CA125, and thyroid transcription factor-1 (TTF-1) can help to determine the origin of an adenocarcinoma or aid in the recognition of other tumors. In patients who do not have an obvious primary site of disease or screening radiographs, these new markers can help focus the search for and guide the treatment of the underlying lesion. CA125 is positive in patients with ovarian cancer, CK7 is positive in patients with breast and lung carcinoma, and CK20 is indicative of colon carcinoma if the CK7 marker is negative. Gastrointestinal stromal tumor (GIST) is positive for CD117 (c-kit) and CD34, whereas 75% of bronchogenic carcinomas are positive for TTF-1. Histochemical staining of the S100 protein family has been used for many years in the diagnosis of malignant melanoma. Recent markers HMB-45, MART-1, and Melan-A have proved to be useful in diagnosis of melanoma. S100B protein has been implicated in downregulation of p53 (oncosuppressor gene). Harpio R, Einarsson R: S100 proteins as cancer biomarkers with focus on S100B in malignant melanoma. Clin Biochem 2004;37:512-518.

TCP is resorbed more rapidly, at a rate of 10 to 20 times faster than hydroxyapatite, partially because its larger pore size makes it a weaker substance. It provides significantly less compressive strength than hydroxyapatite. It does partially convert to hydroxyapatite, thus slowing its resorption rate. The absorbing cell of hydroxyapatite is the foreign body giant cell, not the osteoclast. Optimum pore size appears to be between 150 and 500 um. Lane JM, Bostrom MP: Bone grafting and new composite biosynthetic graft materials. Instr Course Lect 1998;47:525-534.

Chondrocytes are obtained from cartilage harvested from non-weight-bearing areas of the knee. The extracellular matrix is digested, and the chondrocytes are expanded for later transplantation. Cells implanted into a defect are secured with a flap of periosteum. Cells are expanded to obtain 20 to 50 times the original number of cells to transplant at a cell density of 3x10-7 cells/mL. There is a direct relationship between cell number and biosynthetic activity. Osteochondral lesions of up to 8 mm may be treated with autologous transplant alone; larger depth lesions should be bone grafted at the time of harvest. Mesenchymal stem cells differentiate easily into fibrous tissue, bone, and fat; conversion of mesenchymal stem cells into cartilage in vitro currently is difficult to accomplish. Goldberg and Caplan, however, were able to obtain cartilage repair using mesenchymal stem cells transplanted into defects in rabbits in vivo. In animal studies, fluorescent-labeled cells persist for at least 14 weeks, integrate with the surrounding normal margins, and become part of the repaired tissue replete with sulfated proteoglycans and type II collagen. Brittberg M, Peterson L, Sjogren-Jansson E, et al: Articular cartilage engineering with autologous chondrocyte transplantation. J Bone Joint Surg Am 2003;85:109-115.

Plastic deformation of viscoelastic tissues represents deformation of the soft tissues to the point of failure. The lining up of collagen fibers would be in the "toe region" of the curve, which, in the case of the FSU, would be mainly in the neutral zone. Elastin is a minor contributor to the composition of the ligaments and would be protected by the stiffer collagen fibers. The transition between flexion and extension occurs in the neutral zone, and reversible elongation occurs in the elastic zone. Fardon DF, Garfin SR, Abitbol J, et al (eds): Orthopaedic Knowledge Update: Spine 2. Rosemont, IL, American Academy of Orthopaedic Surgeons, 2002, pp 15-23.

A knock-out mouse to the Vitamin D receptor would cause loss of vitamin D function, resulting in rickets. Renal failure would not occur; although Vitamin D is converted from 25 (OH) D to 1,25 (OH) D in the kidney, the active hormone acts on the gut and bone. Osteopetrosis can be seen as the phenotype for the c fos knock-out mouse; the Jansen-type metaphyseal dysplasia phenotype results from overactivation of the PTH/PTHrp receptor. Although compensatory hyperparathyroidism would occur, excessive PTH would not be able to rescue the skeletal loss and instead phosphoturia and phosphotasia would result. Glowacki J, Hurwitz S, Thornhill TS, et al: Osteoporosis and vitamin-D deficiency among postmenopausal women with osteoarthritis undergoing total hip arthroplasty. J Bone Joint Surg Am 2003;85:2371-2377.

The MRI scan demonstrates a grade III MCL tear. Basic science and clinical studies have shown that nonsurgical management is preferred for MCL tears. Functional rehabilitation and early motion have led to consistently better results than has surgical repair.

Intramembranous ossification occurs through the direct formation of bone without the formation of a cartilaginous intermediate. Clinically, both intramembranous and endochondral ossification occur simultaneously during fracture healing; however, the latter is characterized by the differentiation and maturation of chondrocytes, vascular invasion of a hypertrophic cartilage matrix, and bone formation. Collagens type II and X are cartilage specific and would be characteristic of endochondral ossification, not intramembranous ossification. Li J, Sandell LJ: Transcriptional regulation of cartilage-specific genes, in Rosier RN, Evans C (eds): Molecular Biology in Orthoapedics, Rosemont, IL, American Academy of Orthopaedic Surgeons, 2002, pp 21-24.

Rheumatoid arthritis (RA) is a complex multisystem disorder. It has been suggested that patients with RA have an impaired capacity to control infection with Epstein-Barr virus. Epstein-Barr virus has oncogenic potential and is implicated in the development of some lymphomas. Recent publications provide evidence for an altered Epstein-Barr virus-host balance in patients with RA who have a relatively high Epstein-Barr virus load. Large epidemiologic studies confirm that lymphoma is more likely to develop in patients with RA than in the general population. The overall risk of development of lymphoma has not risen with the increased use of methotrexate or biologic agents. Histologic analysis reveals that most lymphomas in patients with RA are diffuse large B cell lymphomas, a form of non-Hodgkin lymphoma. Epstein-Barr virus is detected in a proportion of these. Patients with RA do not have prevalence for infection with any of the other mentioned viruses. Callan MF: Epstein-Barr virus, arthritis, and the development of lymphoma in arthritis patients. Curr Opin Rheumatol 2004;16:399-405.