AAOS Orthopedic Basic Science MCQs (Set 2): Bone Biology, Cartilage & Tendon Healing | 2026 Board Review

Zirconia as a bearing surface is "metastable," meaning that, in the microstructure of the material the zirconia molecules are ordered in a tetragonal fashion, but they can easily transform to a monoclinic microstructure that is less wear resistant. Transformation can occur with input of enough energy (eg, thermal energy imparted by steam sterilization or mechanical energy at the bearing surface). Zirconia was introduced as an alternative to alumina because it has a higher toughness, making it less susceptible to gross fracture (ceramics do not undergo fatigue fracture, but rather fail from a process of slow crack growth). Zirconia is highly biocompatible (as are many ceramics) and is essentially immune to corrosive processes that can plague metallic alloys such as stainless steel. Clarke IC, Manaka M, Green DD, et al: Current status of zirconia used in total hip implants. J Bone Joint Surg Am 2003;85:73-84. Haraguchi K, Sugano N, Nishii T, et al: Phase transformation of a zirconia ceramic head after total hip arthroplasty. J Bone Joint Surg Br 2001;83:996-1000.

Complete rupture of the PCL leads to increased contact pressures in the patellofemoral and medial compartments of the knee. However, whether degenerative arthritis will develop and in which compartments still remains controversial.

Removal of more than 50% of a facet joint can lead to segmental instability and compromises the overall strength of the joint. Removal of the lamina, interspinous ligament, and ligamentum flavum are standard features of a cervical laminectomy. Most surgeons favor fusion with instrumentation of a laminectomized cervical spine. If the anterior part of the spine is already ankylosed from previous surgery or from degenerative conditions, or a posterior fusion with instrumentation is included, then the risk of kyphosis or instability is reduced.

Changing from a solid keel to a keel with a sagittal split allows an amputee to navigate uneven terrain more easily. Changing the length of the keel affects the responsiveness of the prosthesis but does not address the surface conditions for ambulation. The SACH is not used as frequently anymore, because overload problems to the nonamputated foot have been observed. Koval K (ed): Orthopaedic Knowledge Update 7. Rosemont, IL, American Academy of Orthopaedic Surgeons, 2002, pp 31-45.

Adult articular cartilage has less than 5% of the synthesis rate of type II collagen than that seen in developing teenagers. Both synthesis and degradation of type II collagen in normal adult articular cartilage is very low compared to children. In osteoarthrosis, both synthesis and degradation are increased, but the collagen does not properly incorporate into the matrix. Lippiello L, Hall D, Mankin HJ: Collagen synthesis in normal and osteoarthritic human cartilage. J Clin Invest 1977;59:593-600.

Transcription factors regulate the activation or repression of cartilage-specific genes. Sox-9, considered a major regulator of chondrogenesis, regulates several cartilage-specific genes during endochondral ossification, including collagen types II, IV, and XI and aggrecan. Li J, Sandell LJ: Transcriptional regulation of cartilage-specific genes, in Rosier RN, Evans C (eds): Molecular Biology in Orthoapedics, Rosemont, IL, American Academy of Orthopaedic Surgeons, 2002, pp 21-24.

The vascular supply to the medial and lateral menisci originates predominantly from the medial and lateral genicular arteries. The popliteal artery splits into the superior genicular, which splits into medial and lateral branches supplying the patellar cartilage and the posterior cruciate ligament. The middle genicular artery also supplies the anterior curciate ligament, posterior cruciate ligament, and collateral ligaments. The inferior genicular splits into medial and lateral branches and supplies the menisci and other knee ligaments. Despite propagation of incorrect terminology, there is no superior or lateral genicular artery.

Contact between metals in a biologic environment leads to galvanic corrosion. Reduction potentials of Co-Cr and stainless steel produce the worst combination of metals in commonly used implants. Because the fixation implants are not intended to contact the existing implant, it is not as great a consideration as the plate and the screws and cables that will directly contact each other. Miller MD (ed): Review of Orthopaedics, ed 3. Philadelphia, PA, WB Saunders, 2000, pp 119-144.

Muscle contractions can be classified by tension, length, and velocity. Isometric contractions involve changing tension in the muscle while the muscle stays at a constant length. An example would be pushing against a wall. Isokinetic contractions occur when the muscle maximally contracts at a constant velocity. Isotonic contractions involve constant tension throughout the range of motion. Eccentric contraction is when the muscle lengthens during contraction. Eccentric contractions have the highest risk of injury. Plyometrics are eccentric contractions at a rapid rate. Simon SR (ed): Orthopaedic Basic Science. Rosemont, IL, American Academy of Orthopaedic Surgeons, 1994, pp 89-125.

Normal articular cartilage is composed of three zones that are based on the shape of the chondrocytes and the distribution of the type II collagen. The tangential zone has flattened chondrocytes, condensed collagen fibers, and sparse proteoglycan. The intermediate zone is the thickest layer with round chondrocytes oriented in perpendicular or vertical columns paralleling the collagen fibers. The basal layer is deepest with round chondrocytes. The tidemark is deep to the basal layer and separates the true articular cartilage from the deeper cartilage that is a remnant of the cartilage anlage, which participated in endochondral ossification during longitudinal growth in childhood. The tidemark divides the superficial uncalcified cartilage from the deeper calcified cartilage and also is the division between nutritional sources for the chondrocytes. The tidemark is the zone in which chondrocyte renewal took place in childhood. The tidemark is found only in joints and not in the cap of an enchondroma. It is seen most prominently in the adult, nongrowing joint.

Growth factors are proteins secreted by cells that can act on target cells to produce certain biologic actions. These actions can be described as autocrine, paracrine, and endocrine. Autocrine actions are those in which the growth factor influences an adjacent cell of its origin or identical phenotype. Paracrine actions are those in which the protein influences an adjacent cell that is different in its origin or phenotype. Endocrine actions are those in which the factor influences a cell located at a distant anatomic site. Lieberman J, Daluiski A, Einhorn TA: The role of growth factors in the repair of bone: Biology and clinical applications. J Bone Joint Surg Am 2002;84:1032-1044.

Cancellous bone strength and stiffness are determined primarily by the apparent density (the amount of bone per unit volume). Strength varies approximately as the square of the density, and stiffness as the cube of the density; therefore, these are very strong relationships. Cancellous bone strength also depends on the mineral content, the rate of loading (it is viscoelastic), the anatomic level, and the trabecular number (an histomorphometry term), but all to a markedly lesser extent than density. Carter DR, Hayes WC: The compressive behavior of bone as a two-phase porous structure. J Bone Joint Surg Am 1977;59:954-962.

Patients with an above-the-knee amputation have a 65% increase in energy expenditure. A patient with a transtibial amputation requires 25% more energy above baseline values; however, bilateral transtibial amputations are associated with a 40% increase in energy expenditure. Otis JC, Lane JM, Kroll MA: Energy cost during gait in osteosarcoma patients after resection and knee replacement and after above-the-knee amputation. J Bone Joint Surg Am 1985;67:606-611.

Bacitracin is a polypeptide obtained from a strain (Tracy strain) of Bacillus subtilis. It is stable and poorly absorbed from the intestinal tract; its only use is for topical application to skin, wounds, or mucous membranes. Concentrations of 500 to 2,000 units per milliliter of solution or gram of ointment are used for topical application. Bacitracin is mainly bactericidal for gram-positive bacteria, including penicillin-resistant staphylococci. In combination with polymixin B or neomycin, bacitracin is useful for suppression of mixed bacterial flora in surface lesions. Bacitracin is toxic for the kidney, causing proteinuria, hematuria, and nitrogen retention; therefore, it has no place in systemic therapy. Bacitracin is said not to induce hypersensivity readily, but reactions to this agent have been described. Rosenstein BD, Wilson FC, Funderburk CH: The use of bacitracin irrigation to prevent infection in postoperative skeletal wounds: An experimental study. J Bone Joint Surg Am 1989;71:427-430.

Ceramics have the following properties: They are resorbed at varying rates, and the chemical composition of the ceramic significantly affects the rate of resorption. For example, tricalcium phosphate (TCP) undergoes biologic resorption 10 to 20 times faster than hydroxyapatite. The partial conversion of TCP to hydroxyapatite once it is in the body significantly reduces the rate of resorption. Some segments of hydroxyapatite can remain in place in the body for 7 to 10 years. In clinical trials, TCP more readily remodels because of its porosity, but it is weaker. The success of converted corals as a bone graft substitute relies on a complex sequence of events of vascular ingrowth, differentiation of osteoprogenitor cells, bone remodeling, and graft resorption occurring together with host bone ingrowth into and on the porous coralline microstructure or voids left behind during resorption. Lane JM, Bostrom MP: Bone grafting and new composite biosynthetic graft materials. Instr Course Lect 1998;47:525-534. Walsh WR, Chapman-Sheath PJ, Cain S, et al: A resorbable porous ceramic composite bone graft substitute in a rabbit metaphyseal defect model. J Orthop Res 2003;21:655-661.

Linazolid is the first agent of the oxazolidinone group of antibiotics and is very active against methicillin-sensitive Staphylococus aureus, S epidermidis, and vancomycin-resistant enterococci. The drug has no gram-negative activity. Linazolid inhibits protein synthesis by blocking formation of the 70S ribosomal translation complex. This mechanism of action is unique to the oxazolidinones. Rybak MJ, Cappelletty DM, Moldovan T, et al: Comparative in vitro activities and postantibiotic effects of the oxazolidinone compounds eperezolid (PNU-100592) and linezolid (PNU-100766) versus vancomycin against Staphylococcus aureus, coagulase-negative staphylococci, Enterococcus faecalis, and Enterococcus faecium. Antimicrob Agents Chemother 1998;42:721-724.

There is a negative change of calcium balance with a decrease in intestinal absorption and an increase in urinary calcium loss. The reduction of intestinal absorption is accompanied by reduced circulating concentrations of total, but not free 1,25-dihydroxyvitamin D. However, estrogen may also directly regulate intestinal calcium resorption independent of vitamin D. Tubular resorption of calcium is higher in the presence of estrogen. Studies of the levels of PTH in the presence of estrogen are controversial. Oh KW, Rhee EJ, Lee WY, et al: The relationship between circulating osteoprotegerin levels and bone mineral metabolism in healthy women. Clin Endocrinol (Oxf) 2004;61:244-249.

The compaction of FDBA is faster than that of fresh-frozen bone. The maximal stiffness reached by both materials when tested was the same (55 MPa), but the FDBA required fewer impactions to achieve that stiffness. Because it is easier to impact, the FDBA may be mechanically more efficient than the fresh-frozen bone in surgical conditions. The brittleness of irradiated FDBA, caused by loss of the capacity to absorb energy in a plastic way, increases the compactness and stiffness of morcellized grafts. The failure rate of fusion in adolescent idiopathic scoliosis has been shown to be much higher in FDBA than in either iliac crest bone graft or composite autograft with demineralized bone matrix. There is a greater erosive surface response to allograft when compared to autograft or frozen allograft, with a larger number of osteoclast and osteoblast nuclei seen microscopically. Cornu O, Libouton X, Naets B, et al: Freeze-dried irradiated bone brittleness improves compactness in an impaction bone grafting model. Acta Orthop Scand 2004;75:309-314. Price CT, Connolly JF, Carantzas AC, et al: Comparison of bone grafts for posterior spinal fusion in adolescent idiopathic scoliosis. Spine 2003;28:793-798.

When a study yields a negative result between treatment groups, the next step is to perform a power analysis. The power, by definition, is the probability of rejecting the null hypothesis: in this example the null hypothesis would be that children treated with bisphosphonates would have fewer fractures than the untreated control population. The power analysis helps answer the question as to whether the null hypothesis should be rejected and the finding is real, or whether the sample size was too small or the effect of treatment too subtle to demonstrate a difference between the treatment and control groups. Buckwalter JA, Einhorn TA, Simon SR (eds): Orthopaedic Basic Science: Biology and Biomechanics of the Musculoskeletal System, ed 2. Rosemont, IL, American Academy of Orthopaedic Surgeons, 2000, p 7.

Water is the most abundant component of articular cartilage with a wet weight of 65% to 80%. Of the water, 80% is at the surface and 65% at the deep zone. Collagen accounts for 10% to 20% of the wet weight, with type II collagen accounting for 90% to 95% of the total collagen content. Small amounts of types V, VI, IX, X, and XI collagen are also present. Proteoglycans comprise 10% to 15% of the wet weight of collagen. The remainder of the wet weight is made up of other collagens, noncollagenous proteins, and chondrocytes.

Tendons are dense, primarily collagenous tissues that attach muscle to bone. Collagen content of the dry weight is slightly greater than that found in ligaments and is predominantly type I. Type III collagen makes up the remaining ~5% of total collagen content. Kasser JR (ed): Orthopaedic Knowledge Update 5. Rosemont, IL, American Academy of Orthopaedic Surgeons, 1996, pp 10-12. Garrett WE, Speer KP, Kirkendall DT (eds): Principles & Practice of Orthopaedic Sports Medicine. Philadelphia, PA, Lippincott Williams & Wilkins, 2000, pp 21-37.

Etanercept is a fusion protein that combines the ligand-binding domain of the TNF-a receptor to the Fc portion of human immunoglobulin G (IgG). Protein serves as a competitive inhibitor of TNF-a signaling. COX2 is the target of NSAIDs, including newer formulations that are more COX2-specific. The remaining responses are not direct targets of etanercept. Weinblatt ME, Kremer JM, Bankhurst AD, et al: A trial of etanercept, a recombinant tumor necrosis factor receptor: Fc fusion protein, in patients with rheumatoid arthritis receiving methotrexate. N Engl J Med 1999;340:253-259.

Fluoroquinolones have been associated with increased rates of tendinitis, with special predilection for the Achilles tendon. Tenocytes in the Achilles tendon have exhibited degenerative changes when viewed microscopically after fluoroquinolone administration. Recent clinical studies have shown an increased relative risk of Achilles tendon rupture of 3.7. The other listed drugs have no known increase in tendon rupture rates nor tendinitis. van der Linden PD, van de Lei J, Nab HW, et al: Achilles tendinitis associated with fluoroquinolones. Br J Clin Pharmacol 1999;48:433-437. Bernard-Beaubois K, Hecquet C, Hayem G, et al: In vitro study of cytotoxicity of quinolones on rabbit tenocytes. Cell Biol Toxicol 1998;14:283-292.

A number of bioabsorbable polymers are used in orthopaedic applications, and all have in common reports of foreign body reactions, which occur in more than 50% of patients in some series. In general, the high cost of these polymers is offset by the elimination of a second surgery to remove the implant. Bioabsorbable polymers are low strength in comparison to metallic alloys but of sufficient strength for many orthopaedic applications. The elastic modulus is not as high as many other orthopaedic biomaterials, making them suitable for applications where lower stiffness is an asset. Ambrose CG, Clanton TO: Bioabsorbable implants: Review of clinical experience in orthopedic surgery. Ann Biomed Eng 2004;32:171-177.

The superficial portion of the MCL contributes 57% and 78% of medial stability at 5 degrees and 25 degrees of knee flexion, respectively. The deep MCL and posteromedial capsule act as secondary restraints at full knee extension. The anterior cruciate ligament and PCL also provide secondary resistance to valgus loads.