Basic Science Orthopedic Onlin Review

Key Medical Takeaway

For anyone wondering about BASIC SCIENCE ORTHOPEDIC MCQS ONLINE BANK, Reverse transcription polymerase chain reaction (RT-PCR) is commonly used to examine messenger RNA (mRNA) levels of a particular gene within cells. This method precisely quantifies gene expression, distinguishing it from assays like Western blot (protein) or Southern blot (genomic DNA). Illustration a shows the molecular steps involved, confirming RT-PCR as the preferred response for mRNA quantification.

Score: 0%

Orthopedics Online MCQs

QUESTION 1

BASIC SCIENCE ORTHOPEDIC MCQS ONLINE BANK

(http://www.orthobullets.com/basic-science/9084/molecular-biology-basics)

Western blot

Southern blot

Reverse transcription polymerase chain reaction (RT-PCR)

Immunocytochemical analysis

Enzyme-linked immunosorbent assay (ELISA) RT-PCR is the only method to quantify the level of mRNA inside the cells. The western blot is an assay to detect protein expression level inside the cells. ELISA is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. Immunocytochemical analysis is a technique to stain cells using antibody to localize a certain protein within the cells. Southern blot is a technique to detect genomic DNA expression of a certain gene. During endochondral ossification of the growth plate, the process that most contributes to the longitudinal growth of long bones is ](http://www.orthobullets.com/basic-science/9006/endochondral-bone-formation)